| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053927 |
| Chromosome: | chromosome 16 |
| Location: | 4325386 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668950 | CPZ | (1 of 1) 3.4.17.1 - Carboxypeptidase A / Carboxypolypeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACACTGCAGGAGCGCAAGGTAGAAACTTCAGTGCGGGATGGCATTG |
| Internal bar code: | CGGGCGAGGACACATATGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4648 |
| LEAP-Seq percent confirming: | 93.8462 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAACCGCCAGGAAATG |
| Suggested primer 2: | CGAGCGGTCAGTTGGTTAGT |