| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053930 |
| Chromosome: | chromosome 8 |
| Location: | 3421767 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378200 | PPO1 | (1 of 1) 5.1.99.6 - NAD(P)H-hydrate epimerase / NAD(P)HX epimerase; Pyridoxamine 5'-phosphate oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGGGGAGTGGGAGTGGCGGTGGGGCTGCAGCGGCACAAGAGAAGGA |
| Internal bar code: | TGTACTTGCTTCAATTAATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1798 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGGTTGTGCACTACAGA |
| Suggested primer 2: | ACCTGTGTGTGTGTGTGTGT |