Insertion junction: CLIP2.053940_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.053940
Chromosome:chromosome 2
Location:3281203
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097450 (1 of 1) 4.1.2.25 - Dihydroneopterin aldolase / dihydropteridine glycolaldehyde-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGTAGCTGACTGTGTCGGCTAGGTCGTCTGTGGCGCCCGCCTGCAAC
Internal bar code:TTAGACGAATGGCAAGAAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:581
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGAGGTTGGCACAGCAAG
Suggested primer 2:CCAATCCCCAGGTGATTGCT