Insertion junction: CLIP2.053940_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053940
Chromosome:chromosome 2
Location:3281204
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097450 (1 of 1) 4.1.2.25 - Dihydroneopterin aldolase / dihydropteridine glycolaldehyde-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTACAAGTGGGTTATGAGTCACTGGTCTCACCTACAGTCGTTTAATT
Internal bar code:TTAGACGAATGGCAAGAAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3153
LEAP-Seq percent confirming:89.0244
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:CCAATCCCCAGGTGATTGCT
Suggested primer 2:TAGGAGGTTGGCACAGCAAG