| Insertion cassette: | CIB2 | 
| Side of cassette: | 5' | 
| Strand: | + | 
| Strain: | CLIP2.053941 | 
| Chromosome: | chromosome 2 | 
| Location: | 5348224 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre02.g113400 | PACRG1,PACRG,BUG21 | Parkin Co-Regulated Gene Protein; (1 of 1) PTHR21207:SF2 - PARKIN COREGULATED GENE PROTEIN | CDS | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCACGAATCCTGCCTGAATGAGGCACCTGCTGATCACTCCGGCGATG | 
| Internal bar code: | CTTCAAGAACGTGGAGTCCACC | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1959 | 
| LEAP-Seq percent confirming: | 84.6154 | 
| LEAP-Seq n confirming: | 11 | 
| LEAP-Seq n nonconfirming: | 2 | 
| LEAP-Seq n unique pos: | 13 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCTGGCGGTAGTAGGGC | 
| Suggested primer 2: | GCTATGCGCTTGTACTGTGC |