Insertion junction: CLIP2.053942_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.053942
Chromosome:chromosome 17
Location:3107410
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720500 (1 of 5) 2.7.1.68 - 1-phosphatidylinositol-4-phosphate 5-kinase / Type I PIP kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACACGCGAACGCATGGATGGTGATGACAGCAGCTGCGCAACCTCCG
Internal bar code:GTCCGCCTTGTTAATCGATAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1544
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTTGGCGACGATTATGC
Suggested primer 2:GCAGCAACTCAGAGCAAACC