| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053956 |
| Chromosome: | chromosome 13 |
| Location: | 870414 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567450 | HON1 | Histone H1; (1 of 3) K11275 - histone H1/5 (H1_5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCTAAACATGTTGCCTGTCCTGCAGATGGTGGCTGATGCCATCAGCA |
| Internal bar code: | ATAGGGTGGAGGAAAGTGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2548 |
| LEAP-Seq percent confirming: | 73.8636 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTCTTGATGGCCAGCGA |
| Suggested primer 2: | ACGCAAATGGTTGGCTTGTC |