| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053960 |
| Chromosome: | chromosome 12 |
| Location: | 2952600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502251 | (1 of 1) PTHR31065:SF4 - PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTTCCATTTTTGCACGGGTAATGGGGCAGGGACGGCAGGGCAGCTT |
| Internal bar code: | CCTCAGTATCGAGCCAGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1505 |
| LEAP-Seq percent confirming: | 73.8462 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACAACATCATGACCAGC |
| Suggested primer 2: | ATAACCACATCGTCCTGCCC |