| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053965 |
| Chromosome: | chromosome 12 |
| Location: | 9651032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558353 | (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAGAGCGCCTGTGTGGCCCCCGGCCGTCACACGGCCATGTGAAGCAT |
| Internal bar code: | AATGATTCATCGTGTAGCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 2.85714 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTATGGGTATGGAGGTG |
| Suggested primer 2: | TAGTCTCCCGCCAAAACCAC |