Insertion junction: CLIP2.053977_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053977
Chromosome:chromosome 11
Location:392302
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467582 RIB21,FAP306 Ribbon binding 21 kDa microtuble inner protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGACGTGATGTCAGGGCTGCGGTCTCGGGCGAGACAGCGTATTTGGGC
Internal bar code:GCACGTGGCCAAATTCAGAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2073
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCGCTTCTCCATAAAGC
Suggested primer 2:CCGTGTCTGGTACTTTGGCT