| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053996 |
| Chromosome: | chromosome 10 |
| Location: | 1856767 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431050 | SRR13 | (1 of 1) PTHR19331//PTHR19331:SF253 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED; Speract/scavenger receptor, transmembrane glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACCTGCTGTCCGTGAACGGCAGCGGCGCAGGGGCTGTAGCGGGAGC |
| Internal bar code: | ATGGAGCACAGCGGACATTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1286 |
| LEAP-Seq percent confirming: | 89.6552 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACCTATAGGCGCCTAG |
| Suggested primer 2: | TCGTAACATGCCTGACCACC |