| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054003 |
| Chromosome: | chromosome 12 |
| Location: | 3431826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497652 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN | intron | |
| Cre12.g497653 | SNE13,NED1 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Putative Cinnamoyl-CoA reductase/flavanone 4-reductase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAACAAGCCGCATACCGCCGCGGTGGGGCTCTGGCGGGTTGAGGCTT |
| Internal bar code: | AAAGCCATGCAGTCAAGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1361 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGGGCCCATGTCATGAA |
| Suggested primer 2: | CTCTCCCCCACAGCAAGAAG |