Insertion junction: CLIP2.054010_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054010
Chromosome:chromosome 16
Location:918327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648300 PHT6,PHT4-6,PHT4F Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 3) PTHR11662:SF243 - ANION TRANSPORTER 6, CHLOROPLASTIC-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCGCATCCGGCGCCTCGCCATCTGGGTCGGCGGAGGCGGACGCTACA
Internal bar code:TGTCTATAGGTGGGAATCCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:953
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGATAACGCTTCGAGGG
Suggested primer 2:GCCGTTATCCCGATCGACTT