Insertion junction: CLIP2.054019_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054019
Chromosome:chromosome 10
Location:878371
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g423750 COQ3 Hexaprenyldihydroxybenzoate methyltransferase; (1 of 1) 2.1.1.114//2.1.1.222//2.1.1.64 - Polyprenyldihydroxybenzoate methyltransferase / Dihydroxyhexaprenylbenzoate methyltransferase // 2-polyprenyl-6-hydroxyphenol methylase / 2-octaprenyl-6-hydroxyphenol methylase // 3-demethylubiquinol 3-O-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCCTCCCTCTCCCCTCCAACCCACCGGCGCCAGCAGCGACGCCAGCA
Internal bar code:GGGTTTCCGCGTAAAAAGCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1824
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAGATACACGCTCCCCCT
Suggested primer 2:CACACAGAGACACACCTCCC