| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054024 |
| Chromosome: | chromosome 3 |
| Location: | 5154730 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181000 | (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATTGTACTCGGAATGTTGGTGTCAACATACGGAGTGCTTGAACACTG |
| Internal bar code: | TCACCACCGAGGCCCGCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2924 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCCACTACCACATGCT |
| Suggested primer 2: | CTCTCAGATGTTGCATGCGC |