| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054025 |
| Chromosome: | chromosome 12 |
| Location: | 9324522 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542000 | D-tyrosyl-tRNA(Tyr) deacylase; (1 of 1) 3.1.1.96 - D-aminoacyl-tRNA deacylase / D-tyrosyl-tRNA(Tyr) aminoacylhydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCAGCTGATTCCCAGAACCCAGCAGATGACAAACAATTTGCAACGG |
| Internal bar code: | ATGCTTGAGAGGTGGAGATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4102 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTAGACTGCGCGTTAGC |
| Suggested primer 2: | CTTCCCTCGGTCACATGCTT |