| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054028 |
| Chromosome: | chromosome 4 |
| Location: | 2089147 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTATCTAACCCTTCTTGACCGGTCTTCCGCCACTCGAGTTCCCCGCC |
| Internal bar code: | ATGATGCCCTTGACTAAAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTTGCTACGAGGCCTC |
| Suggested primer 2: | CCGTGCAAGCACACATCAAA |