| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054033 |
| Chromosome: | chromosome 12 |
| Location: | 1825447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512500 | (1 of 1) PF07962 - Replication Fork Protection Component Swi3 (Swi3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGCCCTCCGCTAGCTGCTGCTGCTGCTGTTGCTGCTGCGGCCCCGG |
| Internal bar code: | CCGAGAGAGCTCCCTTAGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1086 |
| LEAP-Seq percent confirming: | 21.0526 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTGACCCTCCATGCTCG |
| Suggested primer 2: | ACCATCGGCACACAAGCTAA |