| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.054069 |
| Chromosome: | chromosome 12 |
| Location: | 6200324 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535851 | RPL8 | Cytosolic 80S ribosomal protein L8; (1 of 1) K02938 - large subunit ribosomal protein L8e (RP-L8e, RPL8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGCAATGCCGGCGTTGGGATTAGGCCCTGAGATTTGTGGCAACGGC |
| Internal bar code: | GTCAGCGAGTGGCCCTTGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1321 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCTGTACTGGACGTCA |
| Suggested primer 2: | GCTTGCTGCTAAAAACCGCT |