| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.054113 |
| Chromosome: | chromosome 12 |
| Location: | 5149550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526700 | RBL9 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAACGGGTTGGGCAGCTGCGGCGGACCCGTGGAGGTGGCGGCAGCCA |
| Internal bar code: | ATACCTATAGAACACCCCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1258 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGCTGACGGAAATCGCG |
| Suggested primer 2: | GTTGTGCGTGTGTGTCTTCC |