Insertion junction: CLIP2.054116_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.054116
Chromosome:chromosome 6
Location:3587863
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278128 (1 of 1) IPR000953//IPR005818//IPR016197 - Chromo/chromo shadow domain // Linker histone H1/H5, domain H15 // Chromo domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCATCATCGCCCATCACCCGTTGCGTGAGCGCTGCGGCAAACTCCAGC
Internal bar code:GTTCTATGTCAAATGAACCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1531
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGGAATGACACCCATGAA
Suggested primer 2:CAAAGTGCTTCCAGTTGGGC