Insertion junction: CLIP2.054116_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054116
Chromosome:chromosome 12
Location:5615124
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g531350 CGL62 putative cell cycle associated protein; (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAATGCCTCGACCTGCTCCTCCGTGAGTTGCAGGTGGTTGATATCCCG
Internal bar code:CGTCTTCATCAATACCTGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:730
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGAACTGACTGCCGGTT
Suggested primer 2:TCAAGCGCAATCCATGAGGT