| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054130 |
| Chromosome: | chromosome 12 |
| Location: | 5991439 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534200 | (1 of 1) PTHR24365//PTHR24365:SF381 - TOLL-LIKE RECEPTOR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTATTATCCTGCCCTCTTCCCAGCTGCCCTTACAAGCCTTTCCGCCT |
| Internal bar code: | GGAAGAGAAACACAGGTACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1683 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGGAGGGGATGGGGAAG |
| Suggested primer 2: | CGCCTCCACTGCCACTTTTA |