| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054132 |
| Chromosome: | chromosome 2 |
| Location: | 7304290 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391430 | (1 of 2) IPR015848 - Polyribonucleotide nucleotidyltransferase, RNA-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGTAAGGTGTGCCGAAACCCTTCTCCAGCAGGTCGAGGCACGCCTCG |
| Internal bar code: | CCAATGCTTAATCAGATCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2539 |
| LEAP-Seq percent confirming: | 77.027 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTCCAGCAGGTCAAGG |
| Suggested primer 2: | CTTGTACCCCCTCCCTAGCT |