| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054133 |
| Chromosome: | chromosome 12 |
| Location: | 3046318 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501450 | SDIR1 | RING finger-type E3 ligase, SDIR1 ortholog; (1 of 1) PTHR14155//PTHR14155:SF163 - RING FINGER DOMAIN-CONTAINING // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGATACCGGTACCTGCGTGCGCTCTGTCCACATCTGTCAATGACCGGTG |
| Internal bar code: | ATACAGAGACTGTCCCGCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGCAGCCTCTGTGTTCC |
| Suggested primer 2: | GGTGGGTTCGTCGCTTTAGA |