Insertion junction: CLIP2.054144_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.054144
Chromosome:chromosome 4
Location:3332590
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g227251 (1 of 1) IPR006626//IPR008752//IPR011050//IPR029018 - Parallel beta-helix repeat // Peptidase M11, gametolysin // Pectin lyase fold/virulence factor // Chitobiase/beta-hexosaminidase domain 2-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCACACGCGGCGTGTGTACGGCTTCCTTTATAACAGATACCGGTAAAC
Internal bar code:CCATATCTTTTGTTTATGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1456
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATCCGTAAAATCGTGGC
Suggested primer 2:GCATGAGACACCTGGACCAA