| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054179 |
| Chromosome: | chromosome 10 |
| Location: | 4989132 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTGGGGGGCCTGGCGCCCGCCGGCGGTGGTGCGGCCGGCGTGGGGC |
| Internal bar code: | AGACGCACTGGGATCCTGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCTGAGACGGGTGAAAG |
| Suggested primer 2: | CGCGAGTTTAGACGGTCTGT |