Insertion junction: CLIP2.054194_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.054194
Chromosome:chromosome 11
Location:2110472
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467747 (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCATGCCCCGCTGCGAGGACCCTATCGGCGCAGAGACACGTGCACAC
Internal bar code:GTTTTCTCACACCCTGGTGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1743
LEAP-Seq percent confirming:92.4242
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTCGGACGATGAAGGAG
Suggested primer 2:AGAGGTCCGTAAAAGTGCCG