| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054199 |
| Chromosome: | chromosome 10 |
| Location: | 5451485 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457450 | (1 of 1) PTHR31400:SF1 - PROTEIN GUCD1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCTTGGTGCTGTGGGCGAGCACCCCGAAAGCTCGCTTCCAGAGCTTG |
| Internal bar code: | CGTGCGAAAAAAGTTTAAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3050 |
| LEAP-Seq percent confirming: | 79.4521 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGAAGACTCCCTTGGTC |
| Suggested primer 2: | TAGAAGGACACGTGCAACCC |