| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054203 |
| Chromosome: | chromosome 1 |
| Location: | 2942081 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGTGTCAACTCCCGCTCAACGACTGTGCTATGTCGCTGCCGTGCAC |
| Internal bar code: | ACTTACAGTCTTTACTGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2318 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGCGGTAGGAGATTGGT |
| Suggested primer 2: | TCCTTCATGTCCGTCGGTTG |