Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.054230 |
Chromosome: | chromosome 1 |
Location: | 5030235 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g034900 | CDPK5 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1; Calcium/calmodulin-dependent protein kinase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGACCAGCAGCGTGGCCAACATGCATGCCGCGCGCACCAGCGCGACG |
Internal bar code: | TGATCCGAAGAAGAAGCGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4343 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 77 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTCCGCCACATCACTTGAC |
Suggested primer 2: | GTGATATCTGCCCCTTCGCA |