Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.054234 |
Chromosome: | chromosome 9 |
Location: | 5433992 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g408550 | SAE1 | (1 of 1) K10684 - ubiquitin-like 1-activating enzyme E1 A (UBLE1A, SAE1); SUMO activating enzyme | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCCCCCTGTGAAACCCGCCTGCCTTCACCCGCAGGGTGGCGGAGGC |
Internal bar code: | TGAGTCGGGGGGCATACGCCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 468 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 8 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGAGGGGATAGTGAGGGGG |
Suggested primer 2: | GCTCCACTCCACACACTACC |