Insertion junction: CLIP2.054242_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.054242
Chromosome:chromosome 14
Location:1588616
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g618400 ABCA3 (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCGCCGAAGCTGGCAGCCTGCAGGGTGTAGCGCAAACCTTCTGATGA
Internal bar code:TCTAGGATGGCATCGCGCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2385
LEAP-Seq percent confirming:37.3494
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:52
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:TAATCGTCAACGGGACTGGC
Suggested primer 2:TGATGCTCGATAGGCACGAC