Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.054242 |
Chromosome: | chromosome 14 |
Location: | 1588616 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g618400 | ABCA3 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter 3 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCGCCGAAGCTGGCAGCCTGCAGGGTGTAGCGCAAACCTTCTGATGA |
Internal bar code: | TCTAGGATGGCATCGCGCATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2385 |
LEAP-Seq percent confirming: | 37.3494 |
LEAP-Seq n confirming: | 31 |
LEAP-Seq n nonconfirming: | 52 |
LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAATCGTCAACGGGACTGGC |
Suggested primer 2: | TGATGCTCGATAGGCACGAC |