Insertion junction: CLIP2.054252_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054252
Chromosome:chromosome 2
Location:2533387
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g092284 (1 of 1) 1.13.11.19 - Cysteamine dioxygenase / Persulfurase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTGCAACGCGCCGCACGGGCCACCACGACGGCCCCCACCACTCAGGGG
Internal bar code:CATGAGGGTCCGAGACAACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3461
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGATAGGACGAGGCTGTC
Suggested primer 2:GAACTTGAGAAGTCCGCCGA