| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054271 |
| Chromosome: | chromosome 5 |
| Location: | 2723547 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240400 | SOM2 | (1 of 2) PTHR11746//PTHR11746:SF104 - O-METHYLTRANSFERASE // O-METHYLTRANSFERASE 10; S-adenosyl-L-methionine-dependent O-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACTTAGACCGTAACTACCGTATGCTACATTTGTTATGACGTTTACTT |
| Internal bar code: | ATTGTGTGAATAAAAGTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 17.6471 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACACGGCTAGAGATGG |
| Suggested primer 2: | ATGCAGCTTCAGTCGACCAA |