| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.054273 |
| Chromosome: | chromosome 6 |
| Location: | 3703740 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278154 | (1 of 1) PTHR31560:SF0 - UPF0652 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAAGGGCATGGGAGCCACTTCCAGGGCATCGGGGCCACCGACCCCGT |
| Internal bar code: | GCTGATGCCCAGACCGTTTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3743 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATCTCCACGTCCGTGTG |
| Suggested primer 2: | ATGGAATTGGCGACACGAGT |