Insertion junction: CLIP2.054283_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054283
Chromosome:chromosome 14
Location:1058283
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g614350 (1 of 8) PF00169 - PH domain (PH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATCGTGTGCGGGGCAGGGCACGTGTGCGCCTGTCGGCGCAAGGCGAG
Internal bar code:CAGTGGGACCCGCCGAATCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3017
LEAP-Seq percent confirming:88.2353
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ATAAAAGCCCCCTGCCACTC
Suggested primer 2:CCCCATTATGTTGCGCGAAG