Insertion junction: CLIP2.054287_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.054287
Chromosome:chromosome 2
Location:5172371
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g111950 (1 of 66) 2.7.12.1 - Dual-specificity kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCGCACCTGCAAGTCTGCGACCAAGGGCGCCGCAGTGTTGAGGATGG
Internal bar code:TTTCTACCGCACTGGAAATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:850
LEAP-Seq percent confirming:84.2105
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAGTGTGGTGGATGATGA
Suggested primer 2:CGCTTCGCTTGACTCATTCG