| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054312 |
| Chromosome: | chromosome 3 |
| Location: | 8928446 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204689 | PDE21 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAAGCAACCAGAGATATGGGGATGACGGACTGGACAAGGGGCCACGT |
| Internal bar code: | GTCTAGGGCCTCGATACAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 52.6316 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTGTACTACTACGCCG |
| Suggested primer 2: | CTGTGATCGGGTACGCCAAT |