| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054320 |
| Chromosome: | chromosome 12 |
| Location: | 7730190 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554750 | (1 of 5) IPR000626//IPR019956//IPR029071 - Ubiquitin domain // Ubiquitin // Ubiquitin-related domain; Ubiquitin-like Protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGGAAGTGTGGTCACGGCGATCATTTCTCAGGTCCTCCACGTTTCA |
| Internal bar code: | TTCCAGGGGGTCTTACAAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1555 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGTGGCGTTCAATTATC |
| Suggested primer 2: | ACGGATACCTGAGGTCACGA |