Insertion junction: CLIP2.054359_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.054359
Chromosome:chromosome 2
Location:6508690
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388850 FAP329,ACA1 Flagellar Asociated Protein 329; (1 of 1) PF00122//PF00689//PF00690//PF12710 - E1-E2 ATPase (E1-E2_ATPase) // Cation transporting ATPase, C-terminus (Cation_ATPase_C) // Cation transporter/ATPase, N-terminus (Cation_ATPase_N) // haloacid dehalogenase-like hydrolase (HAD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTGATTCCCGTTCCCCGGACTGTCACTTTCGTCCTGCCCAATGACAA
Internal bar code:TTGTATTAGTTCTATAAAATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2131
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGCTGAGTGGGGTTTGTT
Suggested primer 2:CATACTCCCGCGCACATACT