| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054363 |
| Chromosome: | chromosome 12 |
| Location: | 2178977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509350 | FAP238 | Flagellar Associated Protein 238; (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGCAGAAACTGGACAACCTCAAGCAGAAAGAGGAAGCGCTACGGCA |
| Internal bar code: | TTCATGGATCCGTGTCGCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2185 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCTTGGCCTCGATCTCC |
| Suggested primer 2: | TGCGTAAAAGGCTGGTAGGG |