Insertion junction: CLIP2.054363_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.054363
Chromosome:chromosome 12
Location:2178986
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g509350 FAP238 Flagellar Associated Protein 238; (1 of 140) IPR002048 - EF-hand domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTCCTCAACCTTCTCTGCCTCCTCACGCATCCGCTGCTCCTTCTCAA
Internal bar code:TTCATGGATCCGTGTCGCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1969
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTAAAAGGCTGGTAGGG
Suggested primer 2:CCATCTTGGCCTCGATCTCC