Insertion junction: CLIP2.054367_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054367
Chromosome:chromosome 12
Location:2670215
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505350 FAP10,ACA2 Flagellar Associated Protein 10; (1 of 2) PTHR24093:SF254 - PLASMA MEMBRANE CALCIUM-TRANSPORTING ATPASE 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACCGCGTCATGGACTCCCCCTCCCCTACTCCGAGCTGCTCGCTCAC
Internal bar code:TCGGTCGATAAAATCCACGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2174
LEAP-Seq percent confirming:61.5385
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGGCCAGACGTTTTAGAA
Suggested primer 2:GGAGGCTTACGGACACAACA