Insertion junction: CLIP2.054373_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054373
Chromosome:chromosome 4
Location:2753104
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g223600 (1 of 1) IPR000104//IPR003072//IPR013328 - Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type // 6-phosphogluconate dehydrogenase, domain 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTTTGACGCTGGAGCCCGCCGGACCCCATTGGACACGTTTGTTTGGC
Internal bar code:GGCTAATAGGGTGAAATCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3774
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGACAGGTTCGTCACGA
Suggested primer 2:CCCTGCTGCTCTGAATTGGA