Insertion junction: CLIP2.054390_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054390
Chromosome:chromosome 3
Location:4818020
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g178100 MOT51 (1 of 1) PTHR10877//PTHR10877:SF116 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED; Predicted protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACTCGTCACGCTGGCCCTTCTCGAAGTTGTTGGCGCTATTCTCCAGC
Internal bar code:GAGAGTCGCTGGGTGTTAGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2108
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGTCAACACCAACCAGC
Suggested primer 2:CAGGATGACGGAGATGTCGG