Insertion junction: CLIP2.054391_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054391
Chromosome:chromosome 10
Location:4173771
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g449050 (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGACGCGGCGCGGCGCCTGGGCAGGCGTGGATGGGGGCATGAGGCA
Internal bar code:TTTTCGAGTGAGCATAAACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1188
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGGGCTGCAGAACTCT
Suggested primer 2:GAGGATTATGACGGGCAGGG