Insertion junction: CLIP2.054394_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.054394
Chromosome:chromosome 3
Location:4158536
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g173050 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCGCCCTCGAAACCAAACAACCCTGGTCCGCGACCGCGTGAAGCCAC
Internal bar code:GTGTTAACGGGGCTCGAATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1668
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGCACATCTACATGGAGA
Suggested primer 2:GCTCTATTCTTCCCGAGGCC