| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.054399 |
| Chromosome: | chromosome 6 |
| Location: | 5003088 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCCCGTATCCACACGGCTATAGCCAACGACTCGTCAAAAGTACGCC |
| Internal bar code: | ATTGCTGTTAATAATAGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3341 |
| LEAP-Seq percent confirming: | 97.6923 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCGGTCACGGGAAAAG |
| Suggested primer 2: | TATCTCGCAAGGGTCTGGGA |