Insertion junction: CLIP2.054402_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.054402
Chromosome:chromosome 16
Location:3766102
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g685217 NUP58 Nucleoporin 58; (1 of 1) K14307 - nucleoporin p58/p45 (NUPL1, NUP49) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGCCAGGAACGCCGGGTGCGGGCGGCGCGGGAGCGGGCGGCGGCGCG
Internal bar code:GATCATTTGTCGAGGTGTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1866
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCTTGTTGCTGCTGCTT
Suggested primer 2:CACTGATCTCCCACCCCAAC